Hari Shroff (NIH/NIBIB) et al.,
During acquisition, sample and objective A(B) are held stationary, the light sheet is scanned through the sample using galvanometric mirrors (not shown), and a piezoelectric objective stage moves objective B(A) in sync with the light sheet, ensuring that excitation and/or detection planes are coincident. The sample is mounted onto a rectangular coverslip that is placed onto a 3D translation stage, ensuring correct placement relative to objectives.
Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy.
Nat Biotechnol. 2013 Nov;31(11):1032-8.
Illustrator: Alan Hoofring